https://www.ijmhr.org/ijar.htm editor_anatomy@ijmhr.org
ISSN:       2321-4281 (E) 2321-8967 (P)

IJAR.2019.133

DOWNLOAD PDF
Table of Contents

Type of Article:  Original

Volume 7; Issue 2.1 (April 2019)

Page No.: 6424-6431

DOI: https://dx.doi.org/10.16965/ijar.2019.133

HISTO-HORMONAL STUDIES OF THE EFFECT OF Sida acuta ON THE TESTIS OF ADULT WISTAR RATS

Kebe, E. Obeten *1, Ettah, E. Nkanu 2, Gabriel U. Udo-Affah 3.

*1 Department of Anatomy and Forensic Science, Cross River University of Technology, Okuku, Nigeria.

   2 Department of Physiology, Cross River University of Technolog, Okuku, Nigeria.

   3 Department of Human Anatomy, University of Calabar, Calabar.

Corresponding author: Kebe E. Obeten, Department of Anatomy and Forensic Science, Cross River University of Technology, Okuku, Nigeria. Phone: +2348035505856 E-Mail: fredobeten@yahoo.com

ABSTRACT:

This study was carried out to evaluate the effects of ethanolic leaf extract of Sida acuta on the histology of the testis and reproductive hormones of male adult Wistar rats. Twenty five rats weighing between 180 – 220 g were assigned to five groups (control, olive oil control, low dose, medium dose and high dose) with five animals each. Normal control fed only on rat chow, olive oil control group received 0.5ml of olive oil. The experimental groups, low dose, medium dose and high dose received 500mg/kgBW, 1000mg/kgBW 1500mg/kgBW of the extract orally for 60days respectively. The animals were sacrificed at end of sixty days using chloroform anesthesia. Blood was collected from the left ventricle of the heart through cardiac puncture for hormonal assay. The testes were dissected, sectioned for hematoxylin and eosin (H&E). Hormonal studies showed dose dependent significant (p<0.05) increase in Luteinizing Hormone (LH) across all the treatment groups when compared to the normal and olive oil control groups. Follicle Stimulating Hormone (FSH) was reduced in the low and medium dose animals that received 500mg/kgBw and 1000mg/kgBw of the extract respectively when compared to the control groups, while the high dose group that received 1500mg/kgBw of the extract showed increased FSH level. Testosterone level remained insignificant among all experimental groups when compared to the normal and olive oil control groups. Histological observations of the testes showed dose dependent distortion of the normal cytoarchitecture of the organs, as the high dose (1500mg/kgBw) treated group revealed prominent distortion.  Hence the study suggests that consumption of Sida acuta at high dose may cause adverse effect on reproductive parameters and organs.

Key words: Sida acuta, Luteinizing Hormone, Follicle Stimulating Hormone, Testosterone.

REFERENCES

  1. Tapsell, L. C, Hemphill, I. Cobiac L. Health benefits of herbs and spices: the past, the present, the future. Medical Journal of Australia. 2006;185(4):S4–24.
  2. Lai, P K., Roy, J. Antimicrobial and chemopreventive properties of herbs and spices. Current Medical Chemistry. 2004;11(11):1451–60.
  3. Iwalewa, E. O., Mcgaw, L. J., Naidoo, V. & Eloff, J N. Inflammation: the foundation of diseases and disorders. A review of phytomedicines of South African origin used to treat pain and inflammatory conditions. African Journal of Biotechnology.2007;6:2868-2885.
  4. Ahmed, M. & Hussain, F. Chemical Composition and Biochemical Activity of Aloe Vera (Aloe Barbadensis Miller) Leaves. International Journal of Chemical and Biochemical Sciences. 2013;3(5):29-33.
  5. Agunbiade, O. S., Ojezele, O. M., Ojezele, J. O. & Ajayi, A.Y. Hypoglycaemic activity of Commelina africana and Ageratum conzyzoides in relation to their mineral composition. African Health Sciences, 2012;12(2):198 –203.
  6. Palaksha, M. B. & Ravishankar, K. Phytochemical Screening and Evaluation of in vitro Antibacterial and Antihelminthic Activities of Sida acuta Leaf Extracts. Journal of Chemical and Pharmaceutical Research. 2012;4(11):4757 – 4761.
  7. Adesuyi, A. O., Awosanya, O. A., Adaramola, F. B. & Omeonu A. I. Nutritional and Phytochemical Screening of Aloe barbadensis. Current Research Journal of Biological Sciences. 2011;4(1):4-9.
  8. Wake, R. R. In vitro Antimicrobial Activity of Extracts of Plants of Genus Sida International Journal of Pharmaceutical Research and Development. 2012;3(11):210-214.
  9. Tijjani, M. A., Abdurahman, F. I., Buba, S. W., Mala, G. I., Akan, J. C., Aji, B. M. & Abdullahi A. S. Chemical and Proximate Contents of Methanolic Leaf Extract of Piliostigma thonningii schum (Camel Foot). Journal of Chemical and Pharmaceutical Research. 2012;4(5):2409–2414.
  10. Singh, A. & Singh, S.K. Evaluation of antifertility potential of Brahmi in male mouse. Contraception 2009;79:71-79.
  11. Singh, S.K. & Chakravarty, S. Histologic changes in the mouse testis after bilateral vasectomy. Asian Journal of Andrology. 2000;2:115-120.
  12. Abdulrhman, F., Inyang, S. I., Abbah, J., Binda, L., Amos, S. & Gamanial, K. Effect of aqueous leaf extract of Irvingia fabonesis on gastrointestinal tracts of rodents. Indian Journal of Experimental Biology. 2004;42:787-791.
  13. Barker NM. Luteinizing Hormone Deficiency. 2010; Medscape Reference. 8/31/2010. Available at http://emedicine.medscape.com/article/255046-overview.
  14. Nguyen, H. C. T. Luteinizing Hormone. 2015 Medscape. http://emedicine. medscape.com /article/ 2089268-overview#a30.
  15. B. The Endocrine System: Hypothalamus, Pituitary and Pineal Glands. The Endocrine System, Hormones and Natural Alternatives. 2010;1:2-4.
  16. Peeyush, K., Nitish, K., Devendra, S. T. & Ajay, P. Male hypogonadism: Symptoms and treatment. Journal of Advanced Pharmaceutical Technology and Research. 2010;1(13):297-301.

Cite this article: Kebe, E. Obeten, Ettah, E. Nkanu, Gabriel U. Udo-Affah. HISTO-HORMONAL STUDIES OF THE EFFECT OF Sida acuta ON THE TESTIS OF ADULT WISTAR RATS. Int J Anat Res 2019;7(2.1):6424-6431. DOI: 10.16965/ijar.2019.133