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IJAR.2019.160

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Type of Article:  Original Research

Volume 7; Issue 3.1 (July 2019)

Page No.: 6691-6700

DOI: https://dx.doi.org/10.16965/ijar.2019.160

RESVERATROL REVERSES BRAIN GLUTATHIONE SYSTEM INVOLVED NEURONAL LOSS AFTER IMMOBILIZATION STRESS

T. Kalpana 1, Ramya Sree A *2, B. Naveen Kumar 3.

1 Associate professor, Mamatha Medical College, Khammam, Telangana, India.

*2 Assistant Professor, Department of Anatomy, Mamata Medical College, Khammam, Telangana, India.

3 Associate Professor, Department of Anatomy, Mamata Academy Of Medical Sciences, Bachupally, Hyderabad, Telangana, India.

Corresponding author: Dr. Ramya Sree A, Assistant Professor, Department of Anatomy, Mamata Medical College, Rotary Nagar,Khammam. Telangana – 507002, India. Mobile No: 9885658025 E-Mail: ramya.jerus@gmail.com

ABSTRACT:

Background: This study evaluated the antioxidant effects of Resveratrol on stress-induced neuronal loss in rat brain involving brain glutathione system.

Materials and Methods: The control rats received vehicle while another set of rats received 21 days restraint stress. The third and fourth group received similar intensity of restraint stress as well as either 10 or 20mg/kg dose of resveratrol respectively. The cognitive test included passive avoidance test. This was followed by estimation of reduced glutathione and glutathione reductase enzyme levels in brain homogenate and histomorphological study of hippocampus and medial prefrontal cortex.

Results: Restraint stress has resulted in poor retrieval of learning behaviour and resveratrol has enhanced retrieval of learning behaviour in stressed condition in passive avoidance task. Both reduced glutathione and glutathione reductase levels were reduced after restraint stress and resveratrol at both the doses has normalized their levels. Restraint stress has affected CA3, CA2 and dentate regions of the hippocampus and also medial prefrontal cortex. In all these areas resveratrol has minimized neuronal loss which were due to chronic stress.

Conclusion: From the results of the present study we conclude that stress induced oxidative damage involves brain glutathione system and which in turn could be one of the causes for neuronal loss and resveratrol suggests to protect the brain against stress in rat model.

Key words: Resveratrol, Restraint stress, Reduced glutathione, Glutathione reductase, Hippocampus.

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Cite this article: T. Kalpana, Ramya Sree A, B. Naveen Kumar. RESVERATROL REVERSES BRAIN GLUTATHIONE SYSTEM INVOLVED NEURONAL LOSS AFTER IMMOBILIZATION STRESS. Int J Anat Res 2019;7(3.1):6691-6700. DOI: 10.16965/ijar.2019.160