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IJAR.2019.206

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Type of Article:  Original Research

Volume 7; Issue 3.1 (July 2019)

Page No.: 6722-6726

DOI: https://dx.doi.org/10.16965/ijar.2019.206

COMPARATIVE EVALUATION OF THE HEPATOPROTECTIVE EFFECT OF COSTUS PICTUS D DON METHANOLIC LEAF EXTRACT AND SILYMARIN ON PARACETAMOL INDUCED LIVER DAMAGE IN ALBINO WISTAR RATS

Anitha Nancy 1, Jeneth Berlin Raj *2, Manimekalai K 3.

1 Assistant Professor, Department of Anatomy, Mahatma Gandhi Medical College & Research Institute, Sri Balaji Vidyapeeth’ (Deemed to be university), Puducherry, India.

2 Professor, Department of Physiology, Mahatma Gandhi Medical College & Research Institute, Sri Balaji Vidyapeeth’ (Deemed to be university),  Puducherry, India.

3 Professor, Department of Pharmacology, Mahatma Gandhi Medical College & Research Institute, Sri Balaji Vidyapeeth’ (Deemed to be university), Puducherry, India.

Corresponding Author: Dr. Jeneth Berlin Raj, Professor, Department of Physiology, Mahatma Gandhi Medical College & Research Institute, Sri Balaji Vidyapeeth’ (Deemed to be university), Puducherry, India. E-Mail: ancyjean2010@gmail.com

ABSTRACT:

Background: NSAIDs are the common group of drugs used in self-medication, and this is true for especially Paracetamol (acetaminophen).Although considered safe at therapeutic doses, in overdose, paracetamol causes centrilobular hepatic necrosis which can be fatal. As no data is available on the hepatoprotective effect of Costus pictus D Don, we have made an attempt to investigate the protective effect of Costus pictus D Don leaf extract on paracetamol induced liver damage in rats. The aim of the study is to compare the hepatoprotective effect of methanolic leaf extract of Costus pictus D Don and silymarin on liver damage induced by paracetamol in Wistar rats.

Materials and Methods: 30 Healthy male adult Wistar rats (16 weeks old) weighing > 250g were used for the study. The animals will be maintained in a standard cage under controlled temperature (25+2 °C) and light (12:12 light-dark cycle) in MGMC & RI central animal house. The animals were fed with standard rat pellet and hygienic water ad libitum.30 adult Wistar rats will be randomized into 5 groups with 6 rats each as (Normal control -0.5% carboxymethylcellulose (7 days), Toxic control- 0.5% (7 days)+paracetamol 2g/kg(5th day), Test group I-200 mg/kg methanolic leaf extract+paracetamol 2g/kg(5th day) , Test group II-100 mg/kg methanolic leaf extract+paracetamol 2g/kg(5th day)  & Standard group – silymarin 25mg/kg (7 days) + Paracetamol 2 g/kg (5th day) . The animals were sacrificed on 8th day using sodium pentobarbitone 150mg/kg i.p. serum was sent for biochemical analysis for liver function test. Liver was harvested and a portion was taken for histological examination.

Results: In our study methanolic leaf extract of Costus pictus D Don showed beneficial effect on paracetamol induced liver toxicity which was evident by the significant improvement in liver function test consisting of AST, ALT and ALP in a dose dependent manner which is in consistent with the histological findings.

Conclusions: The study has proved the methanolic leaf extract of Costus pictus D Don posses a significant hepatoprotective activity which was comparable to the standard drug silymarin.

Key words: Costus pictus D Don, Silymarin, Hepatoprotection.

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Cite this article: Anitha Nancy, Jeneth Berlin Raj, Manimekalai K. COMPARATIVE EVALUATION OF THE HEPATOPROTECTIVE EFFECT OF COSTUS PICTUS D DON METHANOLIC LEAF EXTRACT AND SILYMARIN ON PARACETAMOL INDUCED LIVER DAMAGE IN ALBINO WISTAR RATS. Int J Anat Res 2019;7(3.1):6722-6726. DOI: 10.16965/ijar.2019.206