IJAR.2023.109
Type of Article: Original Research
Volume 11; Issue 2 (June2023)
Page No.: 8619-8624
DOI: https://dx.doi.org/10.16965/ijar.2023.109
Resin cast in situ: A technique to demonstrate the vasculature of the Nervous system: In Goat Model
Ravindra Kumar B *1, K Thyagaraju 2, Subhadra Devi Velichety 3.
*1 Assistant Professor, Department of Anatomy, Parul Institute of Medical Sciences & Research, Parul University, Vadodara, Gujarat, India. ORCiD: 0000-0003-0569-1472
2 Assistant Professor, Department of Anatomy, Sri Padmavathi Medical College for Women, SVIMS University, Tirupati, Andhra Pradesh, India. ORCiD: 0009-0006-3244-6308
3 Professor and Head, Department of Anatomy, Apollo Institute of Medical Sciences and Research, Chittoor, Andhra Pradesh, India. ORCiD: 0000-0002-3995-8449
Corresponding Author: Dr. Ravindra Kumar B, Assistant Professor, Department of Anatomy, Parul Institute of Medical Sciences & Research, Parul University, Vadodara, Gujarat, India.
E-Mail: dr.ravindrakumar@gmail.com
ABSTRACT
Background: As Anatomical education advances very rapidly, and at the same time, there is huge demand and scarcity of true biological models, mainly brain tissue. In this scenario, in 1977, Guther Von Hagens worked on plastics and experimented voraciously on diffusing various plastics into large specimens and ultimately succeeded and coined the term “Plastination”. This technique is very popular in Western countries as the latter greatly minimizes the health hazards due to formalin exposure while dealing with biological tissue. In continuation with the advancement of expensive plastination to cost-effective resin casting, especially studies over the complex structures like the brain and spinal cord. In This study, we emphasize the cost-effective Resin cast-in-situ method to demonstrate the vasculature of the brain and spinal cord.
Materials and Methods: After obtaining the prior permission, we collected the Five (5) Goat heads from the slaughterhouse. Following the standard dissection procedure over the neck region, identified and canulated the major neck vessels. Through the vessels normal saline water, formalin, and pigmented resin were administered and preserved the goat head using routine preservation technique. After 36 to 48 hours, the routine dissection was scheduled to expose the brain and upper spinal cord segments, and finding were captured and recorded.
Results and Discussion: The specimens show good penetration of dye in the artery and veins, and it’s easy to appreciate and study the vasculature of the brain and upper spinal cord segments, including the Bastons plexus of veins.
Conclusion: In comparison with regular silicon casting, resin casting is very cost-effective and long-lasting, with good penetration of the resin substance up to the capillary level. Furthermore, similar studies may be conducted using in combination with whole organ plastination using silicon and resin embedding.
KEYWORDS: Resin cast in situ, Brain Vasculature, Plastination, Silicon Casting.
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