IJAR.2024.149
Type of Article: Original Research
Volume 12; Issue 3 (September 2024)
Page No.: 8996-9002
DOI: https://dx.doi.org/10.16965/ijar.2024.149
Histological and Immunohistochemical Study of the Beta Cells In Streptozotocin Induced Diabetic Rats Treated with Cynodon Dactylon Aqueous Extract
Madhan Kumar Soutallu Janakiram 1, Raju Sugavasi *2, Radhakrishna Shetty Kommanda 3, Parthiban Govindarajoo 4, Muthuvel Mani 5.
1 Associate Professor, Department of Anatomy, Faculty of Medicine, Manipal University College Malaysia, Melaka, Malaysia. ORCiD: 0000-0002-1395-6453
*2 Associate Professor, Department of Anatomy, Faculty of Medicine, Manipal University College Malaysia, Melaka, Malaysia. ORCiD: 0000-0002-7799-9786
3 Associate Professor, Department of Anatomy, Faculty of Medicine, Manipal University College Malaysia, Melaka, Malaysia. ORCiD: 0000-0003-3603-7510
4 Senior Lecturer, Department of Anatomy, Faculty of Medicine, Manipal University College Malaysia, Melaka, Malaysia. ORCiD: 0000-0002-1767-7531
5 Senior Lecturer, Department of Anatomy, Faculty of Medicine, Manipal University College Malaysia, Melaka, Malaysia. ORCiD: 0000-0001-6034-3206
Corresponding Author: Dr. Raju Sugavasi, Associate Professor, Department of Anatomy, Faculty of Medicine, Manipal University College Malaysia, Melaka, Malaysia. E-Mail: sugavasi.raju@manipal.edu.my
ABSTRACT
Background: Diabetes mellitus is a chronic metabolic disorder that affects human body in terms of physical, psychological and social health. Traditional medicines have been widely used in treatment of diabetes and its complications. After administrating the aqueous extract of Cynodon dactylon in streptozotocin induced diabetic rats, we evaluated the histological and immunohistochemical effects of the β – cells in the pancreatic islets.
Objectives: The objective of the present study is to investigate the antidiabetic effect of Cynodon dactylon aqueous extract in β – cells of islets, against streptozotocin induced diabetic rats.
Materials and Methods: In this study adult male albino rats were used. Aqueous extract of Cynodon dactylon (500 mg/kg.bw/day) administered orally for 45 days. At the end of experimental period, animals were sacrificed, pancreas was isolated and processed for histological and immunohistochemical analysis.
Results: The pancreas of Cynodon dactylon aqueous extract treated diabetic rats showed improved islet morphology; and the pancreatic islets of the Cynodon dactylon aqueous extract treated diabetic rats showed consistent elevation in the insulin levels from the β – cells. Therefore, the antidiabetic activity of Cynodon dactylon aqueous extract might be due to the compounds present in the Cynodon dactylon aqueous extract which stimulate the insulin secretion and protect the intact functional β – cells from streptozotocin induced destruction.
Conclusion: Cynodon dactylon aqueous extract increased the insulin secreting β – cells in the pancreatic islets and protecting the intact β – cells from oxidative damage.
Keywords: Cynodon dactylon, streptozotocin, Pancreatic islets, oxidative stress.
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