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IJAR.2017.237

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Type of Article:  Original Research

Volume 5; Issue 2.3 (June 2017)

Page No.: 3988-3992

DOI: https://dx.doi.org/10.16965/ijar.2017.237

AN INNOVATIVE METHOD TO PREPARE FORMALIN FREE MODELS BY PARAFFIN IMPREGNATION OF HUMAN ORGANS- HEART, BRAIN, SPLEEN AND LIVER

L.K. Jain 1, Hitesh Babel *2.

1 Professor and Head, Department of Anatomy, Geetanjali Medical College and Hospital, Near Eklingpura Circle, Manwakhera, Udaipur, Rajasthan, India.

*2 Professor, Department of Anatomy, Geetanjali Medical College and Hospital, Near Eklingpura Circle, Manwakhera, Udaipur, Rajasthan, India.

Address for Correspondence: Dr. Hitesh Babel, Professor, Department of Anatomy, Geetanjali Medical College and Hospital, Near Eklingpura Circle, Manwakhera, Udaipur, Rajasthan, India. E-Mail: hit_19c@rediffmail.com

ABSTRACT:

Background: Although formalin preserved organs in dissection- hall are used for teaching purpose but the odour and carcinogenic effect of formalin has made us to search for some alternate method for preparing teaching models. One of such methods is plastination but it requires well equipped laboratory and costly chemicals. So our objective was to develop a cheap and easy and feasible method of preparing formalin free teaching models of organs.

Materials and Methods: Heart with great vessels, brain, spleen and liver were removed from cadavers and a fine dissection was done to clear arteries and veins. Organs were washed thoroughly in running tap water to remove small blood clots and formalin. Volume of each organ was measured and recorded in millilitres (ml) by dipping the fresh organ in water and raised of water column was measured. Organs were then dehydrated in three subsequent change of absolute alcohol. Each bath lasted for 3-7 days according to size of organs. They were then put in two subsequent baths of xylene; each lasted for 1-3 days. After xylene bath organs were put in melted wax at 65 degree Celsius. Three changes of wax penetrated the tissue. Again volumes were taken by the same method and recorded. They were coloured for a better differentiation. Varnish paint was applied on the prepared models. Dry and formalin free models were thus ready for demonstration and study purposes.

Results: Models maintained their original shape and had shown shrinkage of 04.54 percent in heart, 43.7 percent in one half of brain, 38.4 percent in spleen, and 47 percent in liver. Results of this method were highly appreciable.

Conclusion: This method was helpful in preservation of valuable human organs in their original shape like a bone for the study of students. Models prepared with this technique were lively in appearance; odour free and without any carcinogenic property so therefore could be safely used for study purposes. Teaching models prepared with this technique no longer needed any preservative material for their storage.

KEY WORDS: Anatomy, Museum, Cadaver, Teaching Model, Wax.

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Cite this article: L.K. Jain, Hitesh Babel. AN INNOVATIVE METHOD TO PREPARE FORMALIN FREE MODELS BY PARAFFIN IMPREGNATION OF HUMAN ORGANS- HEART, BRAIN, SPLEEN AND LIVER. Int J Anat Res 2017;5(2.3):3988-3992. DOI: 10.16965/ijar.2017.237