IJAR.2022.178

Type of Article:  Original Research

Volume 10; Issue 3 (September 2022)

Page No.: 8430-8438

DOI: https://dx.doi.org/10.16965/ijar.2022.178

Efficacy of Embalming Mixture Containing Formalin, Methanol and Water on Early Stage of Decomposition: A Validation Study

Darlington Nnamdi Onyejike *1, Darlington Cyprain Akukwu 2, Odinakachi Ijike 1, Ifeoma Miracle Onyejike 1, Ugochukwu Samuel Aguwa 1, Emeka Ambrose Okubike 1, Emmanuel Nzube Ezenwatu 1, Gloria Chinenye Ojemeni 1, Somadina Nnamdi Okeke 1, Ambrose Echefulachi Agulanna 1.

1 Department of Anatomy, Faculty of Basic Medical Sciences, Nnamdi Azikiwe University, Nnewi campus, Anambra state, Nigeria.

2 Department of Anatomy, Faculty of Basic Medical Sciences, Imo state University, Owerri, Imo state, Nigeria.

Corresponding author: Dr Darlington Nnamdi Onyejike, Department of Anatomy, Faculty of Basic Medical Sciences, Nnamdi Azikiwe University, Nnewi campus, Anambra state, Nigeria. Telephone number: +2348061131867 E-Mail: dn.onyejike@unizik.edu.ng

ABSTRACT

Background: Stabbing with kitchen knife is a common method of homicide in Nigeria; and embalming of decomposing stabbed cadavers has been a challenge for morticians in Nigeria, because of the impairment of embalming fluid through the damaged arteries. The aim of this study is to investigate the effectiveness of an embalming mixture containing formalin, methanol and water on stabbed early decomposing carcasses using porcine analogues.

Methods: Two infant pigs were used for this study. Animals were sacrificed and allowed to reach the early stage of decomposition before embalming. Cervical arterial and hypodermic embalming techniques were employed. Post-embalming changes were observed and scored. A Pearson correlation analysis was used to determine the relationship between the outcome of embalmment and the embalming mixture.

Results: There was a statistically significant moderate positive correlation (r = .576, n = 42, p = .001) between outcome of embalming, and duration of embalming; a statistically significant strong negative correlation (r = -.623, n = 42, p = .001) with volume of embalming fluid; and a statistically significant moderate negative correlation (r = -.528, n = 42, p = .001) with room temperature. There was a statistically insignificant very weak positive correlation (r = .186, n = 42, p = .239) between outcome of embalming and humidity; and a statistically insignificant very weak negative correlation (r = -.141, n = 42, p = .375) with atmospheric temperature.

Conclusions: This study validated the embalming methodology and fluid suitable for arresting early decomposition process of stabbed infant carcasses in Nigeria. More so, an embalming mixture containing formalin, methanol and water in equal proportion is suitable for arresting early decomposition of stabbed cadavers.

KEYWORDS: Embalming of decomposing bodies, Embalming of stabbed bodies, Funeral services, Modern embalming science, Commercial embalming, Stab wounds, Specialist embalming.

REFERENCES

[1]. Mayer RG. Embalming: History, Theory and Practice. 3rd Ed. New York, USA: McGraw-Hill; 2012. p. 10 – 35.
[2]. Brenner E. Human body preservation–old and new techniques. J Anat. 2014; 224 (3): 316 – 344.
[3]. Campobasso CP, Di Vella G, Introna F. Factors affecting decomposition and Diptera colonization. Forensic Sci Int. 2001; 120 (1–2): 18 – 27.
[4]. Mittal P, Malik AK, Khanagwal VP, Paliwal PK. Stabbed, Stabbed,… and Strangulated: Multiple Means of Homicide – A Case Report. Anil Aggrawal’s Internet Journal of Forensic Medicine and Toxicology. 2014; 15 (1): 4.
[5]. Stanley SA, Hainsworth SV, Rutty GN. How taphonomic alteration affects the detection and imaging of striations in stab wounds. Int J Legal Med. 2018; 132: 463–475.
[6]. Bajracharya, S. & Magar, A. (2006) Embalming: An art of preserving human body. Kathmandu Univ Med J. 2006; 4 (16): 554 – 557.
[7]. Onyejike D, Ukoha U, Aladeyelu S. Evaluating the Embalming Procedures of Putrefying Bodies in Relation to Gender in Anambra State, Nigeria. IISJ. 2018; 2 (6): 213 – 222.
[8]. Richins C, Roberts E, Zeilmann J. Improved fluids for anatomical embalming and storage. Anat Rec. 1963; 146: 241 – 243.
[9]. Omil F, Mendez D, Vidal G, Mendez R, Lema J. Toxicity and anaerobic biodegradation of formaldehyde. Enzyme Microb Technol. 1998; 27: 172 – 180.
[10]. McGwin G, Lienert J, Kennedy JI. Formaldehyde exposure and asthma in children: A systematic review. Environ Health Perspect. 2009; 118 (3): 313 – 317.
[11]. Hammer N, Loffler S, Feja C, Sandrock M, Schmidt W, Bechmann I, Steinke H. Ethanol-glycerin fixation with thymol conservation: a potential alternative to formaldehyde and phenol embalming. Anat Sci Educ. 2012; 5: 225 – 233.
[12]. Treichel JL, Murray TG, Lewandowski MF, Stueven HA, Eells JT, Burke JM. Retinal toxicity in methanol poisoning. Retina. 2004; 24 (2): 309 – 312.
[13]. Onyejike D. Comparison of the decomposition timeline of Sus scrofa domestica in rainforest and savannah vegetations of Nigeria [dissertation]. Calabar, Nigeria: University of Calabar; 2021.
[14]. Khalid R, Knowles T, Wotton S. A comparison of blood loss during the Halal slaughter of lambs following Traditional Religious Slaughter without stunning, Electric Head-Only Stunning and Post-Cut Electric Head-Only Stunning. Meat Sci. 2015; 110 (2015): 15 – 23.
[15]. Kelly JA, van der Linde TC, Anderson GS. The Influence of Wounds, Severe Trauma, and Clothing, on Carcass Decomposition and Arthropod Succession in South Africa. Can Soc Forensic Sci. 2011; 44 (4): 144 – 157.
[16]. Bradbury SA, Hoshino K. An improved embalming procedure for long-lasting preservation of the cadaver for anatomical study. Acta Anat (Basel). 1978; 101 (2): 97 – 103.
[17]. Bedino J. Embalming Chemistry: Glutaraldehyde Versus Formaldehyde. Champion Expanding Encyclopedia of Mortuary Practices. 2003; 649: 2614 – 2632.

Cite this article: Darlington Nnamdi Onyejike, Darlington Cyprain Akukwu, Odinakachi Ijike, Ifeoma Miracle Onyejike, Ugochukwu Samuel Aguwa, Emeka Ambrose Okubike, Emmanuel Nzube Ezenwatu, Gloria Chinenye Ojemeni, Somadina Nnamdi Okeke, Ambrose Echefulachi Agulanna. Efficacy of Embalming Mixture Containing Formalin, Methanol and Water on Early Stage of Decomposition: A Validation Study. Int J Anat Res 2022;10(3):8430-8438. DOI: 10.16965/ijar.2022.178