IJAR.2023.101
Type of Article: Original Research
Volume 11; Issue 2 (June2023)
Page No.: 8593-8599
DOI: https://dx.doi.org/10.16965/ijar.2023.101
Histological Features of Different Organs Before and After Treatment of Diabetes by Using Avocado Extract in Rats
Ali A. Tala’a *1, Rafid Khalid Ali 2, Lubna Dhari Mohammed 3 Reyam Abdul Khuder Mohammed 4.
*1 University of Fallujah, College of Veterinary Medicine, Baghdad, Iraq. https://orcid.org/0000-0002-1035-4051
2 University of Fallujah, College of Veterinary Medicine, Baghdad, Iraq. https://orcid.org/0000-0002-8023-4186
3 Mustansiriyah University, Baghdad, Iraq. https://orcid.org/0000-0003-3628-6680
4 Al-Mustaqbal University College, Hilla, Iraq. https://orcid.org/0000-0001-8545-5706
Corresponding Author: Ali A. Tala’a, University of Fallujah, College of Veterinary Medicine, Baghdad, Iraq. E-Mail: alialrawi@uofallujah.edu.iq
ABSTRACT
Aim of the study: The present study aimed to study the histological features of different organs before and after the treatment of diabetes by using avocado extract in rats.
Materials and methods: Hot aqueous extraction of avocado was performed. Forty male rats (weighted 140–190g) were used in this study. Following the time of acclimatization, the animals had an overnight fast of 18 hours before being prepped for alloxan monohydrate-induced diabetes. Before and after induction, measurements of the animals’ body weights and blood glucose levels were made. However, rats received an IP injection of alloxan 150 mg/kg bw. Following that, the rats’ blood glucose levels were tracked every day for 3 weeks to establish stable levels of blood glucose. The animals were divided into 4 groups: Group 1 got water as a non-induced (negative control) condition. Alloxan-induced rats in Group 2 received water as a positive control. Group 3: Alloxan-induced and aqueous extract-treated animals and Group 4 was only given a 40 g/L dose of the aqueous extract of avocado. Pancreas, livers, as well as kidneys from control, alloxanized, and treated rats were taken at different times, processed, and utilized for histological examination after being preserved in 10% formaldehyde till processing and staining.
Results: The current results showed a significant difference between different groups especially in G3 at different weeks. However, rats in G2 exhibited depleted islet cells and regions of cell necrosis. The tiny, preserved islet cells (PIL) in diabetic rats treated with extract after 1 week (G3) were an improvement as compared with rats at G2. As the days advanced, more improvements were seen in the pancreatic architecture of the rats treated with extract, including the presence of more noticeable islet cells and exocrine cells. As seen by the intact pancreatic islet in G3, caused the healing of the pancreatic tissue after 3 weeks of treatment by extracts. Alloxanized rats (G2) showed the presence of cell necrosis as well as infiltrations of inflammatory cells. However, as the course of therapy continued, it became clear that the tissue architecture had improved, and more glomeruli were seen as well as fewer inflammatory cells (G3). Livers of rats in (G2) showed visible cell necrosis, when compared to the histology of G1 and G4. After receiving medication, rats in group G3 had compact, healthy liver tissues after three weeks.
In conclusion, the pancreas, kidneys, and liver were all protected by the avocado extract and showed enhancement in the histological architecture and glucose levels.
Keywords: Diabetes, Avocado, Histology, Rats.
REFERENCES
[1]. Atlas D. International diabetes federation. IDF Diabetes Atlas, 7th edn. Brussels, Belgium: International Diabetes Federation. 2015;33:2.
[2]. Carracher AM, Marathe PH, Close KL. International diabetes federation 2017. https://onlinelibrary.wiley.com/doi/full/10.1111/1753-0407.12644
[3]. AhbiRami R, Zuharah WF. School-based health education for dengue control in Kelantan, Malaysia: Impact on knowledge, attitude and practice. PLoS neglected tropical diseases. 2020 Mar 27;14(3):e0008075. https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0008075
[4]. Noubiap JJ, Nansseu JR, Endomba FT, Ngouo A, Nkeck JR, Nyaga UF, Kaze AD, Bigna JJ. Active smoking among people with diabetes mellitus or hypertension in Africa: a systematic review and meta-analysis. Scientific reports. 2019 Jan 24;9(1):1-1. https://link.springer.com/content/pdf/10.1038/s41598-018-37858-z.pdf
[5]. Chukwuonye II, Chuku A, John C, Ohagwu KA, Imoh ME, Isa SE, Ogah OS, Oviasu E. Prevalence of overweight and obesity in adult Nigerians–a systematic review. Diabetes, metabolic syndrome and obesity: targets and therapy. 2013 Jan 22:43-7.
https://www.tandfonline.com/doi/full/10.2147/DMSO.S38626
[6]. Oyewole OO, Odusan O, Oritogun KS, Idowu AO. Physical activity among type-2 diabetic adult Nigerians. Annals of African medicine. 2014 Oct 1;13(4):189. https://annalsafrmed.org/article.asp?issn=1596-3519;year=2014;volume=13;issue=4;spage=189;epage=194;aulast=Oyewole
[7]. Ugwu E, Adeleye O, Gezawa I, Okpe I, Enamino M, Ezeani I. Burden of diabetic foot ulcer in Nigeria: current evidence from the multicenter evaluation of diabetic foot ulcer in Nigeria. World journal of Diabetes. 2019 Mar 3;10(3):200. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6422858/
[8]. Ahmad SS, Ahmad K, Lee EJ, Lee YH, Choi I. Implications of insulin-like growth factor-1 in skeletal muscle and various diseases. Cells. 2020 Jul 24;9(8):1773. https://www.mdpi.com/2073-4409/9/8/1773
[9]. Mahood AK. Histological study of the effect of Nigella sativa on diabetic nephropathy in rats. Tikrit Medical Journal. 2012 May 1;18. https://www.iasj.net/iasj/download/e8f7607d2a7c2e82
[10]. Farid A, Haridyy H, Ashraf S, Ahmed S, Safwat G. Co-treatment with grape seed extract and mesenchymal stem cells in vivo regenerated beta cells of islets of Langerhans in pancreas of type I-induced diabetic rats. Stem Cell Research & Therapy. 2022 Dec;13(1):1-8.
https://stemcellres.biomedcentral.com/articles/10.1186/s13287-022-03218-y
[11]. El-Kordy EA, Alshahrani AM. Effect of genistein, a natural soy isoflavone, on pancreatic β-cells of streptozotocin-induced diabetic rats: Histological and immunohistochemical study. Journal of microscopy and ultrastructure. 2015 Sep 1;3(3):108-19.
https://www.sciencedirect.com/science/article/pii/S2213879X15000309
[12]. Barbosa AP, Silveira GD, de Menezes IA, Rezende Neto JM, Bitencurt JL, Estavam CD, de Lima AD, Thomazzi SM, Guimaraes AG, Quintans-Junior LJ, dos Santos MR. Antidiabetic effect of the Chrysobalanus icaco L. aqueous extract in rats. Journal of medicinal food. 2013 Jun 1;16(6):538-43. https://www.liebertpub.com/doi/abs/10.1089/jmf.2012.0084
[13]. Umar AZ, Umar UA, Dambazau SM, Sulaiman A. Comparative hepatotoxic effects of aqueous and phenolic extracts of avocado (Persea americana) seed in Wistar albino rats. International Journal of Biochemistry Research & Review. 2016 Jan 1;10(4):1.
http://www.journalrepository.org/media/journals/IJBCRR_3/2016/Feb/Dambazau1042015IJBCRR23196.pdf
[14]. Alhassan AJ, Sule MS, Atiku MK, Wudil AM, Abubakar H, Mohammed SA. Effects of aqueous avocado pear (Persea americana) seed extract on alloxan induced diabetes rats. Greener J Med Sci. 2012;2(1):5–11. https://pdfs.semanticscholar.org/9cd5/9233ed2b494e0051efc4366f0b03ccf87bf0.pdf
[15]. Tabeshpour J, Razavi BM, Hosseinzadeh H. Effects of avocado (Persea americana) on metabolic syndrome: A comprehensive systematic review. Phytotherapy research. 2017 Jun;31(6):819-37. https://onlinelibrary.wiley.com/doi/abs/10.1002/ptr.5805
[16]. Ezejiofor AN, Okorie A, Orisakwe OE. Hypoglycaemic and tissue-protective effects of the aqueous extract of Persea americana seeds on alloxan-induced albino rats. The Malaysian journal of medical sciences: MJMS. 2013 Oct;20(5):31. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3957359/
[17]. Mahadeva RUS, Mainul H, Atif AB. Insulin Stimulative and Anti-Oxidative Effects of Persea americana Fruit Extract on Streptozotocin Induced Hyperglycemic Rats. J Med Biol Sci. 2011;4(1):1–10 https://pubmed.ncbi.nlm.nih.gov/22467999/
[18]. Uchenna UE, Shori AB, Salihin Baba A. Inclusion of avocado (Persea americana) seeds in the diet to improve carbohydrate and lipid metabolism in rats. Revista argentina de endocrinología y metabolismo. 2017 Sep;54(3):140-8.
http://www.scielo.org.ar/scielo.php?pid=S1851-30342017000300006&script=sci_abstract&tlng=en
[19]. Kouamé NG, Koffi C, N’Zoué KS, Yao N, Doukouré B, Kamagaté M. Comparative antidiabetic activity of aqueous, ethanol, and methanol leaf extracts of Persea americana and their effectiveness in type 2 diabetic rats. Evidence-Based Complementary and Alternative Medicine. 2019 Oct 16;2019. https://www.hindawi.com/journals/ecam/2019/5984570/
[20]. Airaodion AI, Ogbuagu EO, Ekenjoku JA, Ogbuagu U, Okoroukwu VN. Antidiabetic effect of ethanolic extract of Carica papaya leaves in alloxan-induced diabetic rats. American Journal of Biomedical Science & Research. 2019;5(3):227-34. https://biomedgrid.com/pdf/AJBSR.MS.ID.000917.pdf
[21]. Arnarson A, Ramel A, Geirsdottir OG, Jonsson PV, Thorsdottir I. Changes in body composition and use of blood cholesterol lowering drugs predict changes in blood lipids during 12 weeks of resistance exercise training in old adults. Aging clinical and experimental research. 2014 Jun;26:287-92. https://link.springer.com/article/10.1007/s40520-013-0172-0
[22]. Salib JY, Michael HN, Eskande EF. Anti-diabetic properties of flavonoid compounds isolated from Hyphaene thebaica epicarp on alloxan induced diabetic rats. Pharmacognosy Research. 2013 Jan;5(1):22. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3579016/
[23]. Yetendje LC, Njateng GS, Dongmo AJ, Mouokeu RS, Feudjio C, Tamekou SL, Iqbal J. In vivo antidiabetic activity and mechanism of action of three Cameroonian medicinal plant extracts. Int J Res Granthaalayah. 2019 Aug;7:415-30.
https://www.granthaalayahpublication.org/journals/granthaalayah/article/view/IJRG19_A08_2641
[24]. Kong M, Xie K, Lv M, Li J, Yao J, Yan K, Wu X, Xu Y, Ye D. Anti-inflammatory phytochemicals for the treatment of diabetes and its complications: Lessons learned and future promise. Biomedicine & Pharmacotherapy. 2021 Jan 1;133:110975.
https://www.sciencedirect.com/science/article/pii/S0753332220311677
[25]. Jovanovski E, Jenkins A, Vuksan V. Effects of Korean White Ginseng (Panax Ginseng CA Meyer) on vascular and glycemic health in type 2 diabetes: results of a randomized, double blind, placebo-controlled, multiple-crossover, acute dose escalation trial. Clinical nutrition research. 2014 Jul 1;3(2):89-97. https://synapse.koreamed.org/articles/1059785
[26]. Abedi Gaballu F, Abedi Gaballu Y, Moazenzade Khyavy O, Mardomi A, Ghahremanzadeh K, Shokouhi B, Mamandy H. Effects of a triplex mixture of Peganum harmala, Rhus coriaria, and Urtica dioica aqueous extracts on metabolic and histological parameters in diabetic rats. Pharmaceutical biology. 2015 Aug 3;53(8):1104-9. https://www.tandfonline.com/doi/full/10.3109/13880209.2014.960943
[27]. Mardani S, Nasri H, Hajian S, Ahmadi A, Kazemi R, Rafieian-Kopaei M. Impact of Momordica charantia extract on kidney function and structure in mice. Journal of nephropathology. 2014 Jan;3(1):35. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3956906/
