IJAR.2024.104
Type of Article: Original Research
Volume 12; Issue 2 (June 2024)
Page No.: 8903-8909
DOI: https://dx.doi.org/10.16965/ijar.2024.104
Tissue Processing using Microwave Oven: A Boon for Histology Slide Preparation
Dane Chandy 1, Prima Swetha D’Souza *2, Susie Jeyalyn David 3.
1 Assistant Professor, Department of Anatomy, Father Muller Medical College, Mangalore, Karnataka, India. ORCiD: 0000-0003-0800-8894
*2 Assistant Professor, Department of Anatomy, K. S. Hegde Medical Academy, Mangalore, Karnataka, India. ORCiD: 0009-0007-3355-2601
3 Assistant Professor, Department of Anatomy, Sree Mookambika Institute of Medical Sciences, Kulasekharam, Kanyakumari District, Tamilnadu, India. ORCiD: 0000- 0001-6509-8879
Corresponding Author: Dr. Prima Swetha D’Souza, Assistant Professor, Department of Anatomy, K. S. Hegde Medical Academy, Mangalore, Karnataka, India. E-Mail: prima@nitte.edu.in
ABSTRACT
Background: Tissue processing is an important step in histology laboratories. Routine tissue processing is time-consuming. Due to the same time factor, microwave tissue processing is now increasingly being used in many histology and pathology laboratories.
Objective: To compare the time taken to process the tissues using the routine conventional and microwave methods.
Methods: The study was conducted in the Histology laboratory. 100 slides (50 conventional method + 50 microwave method) were processed using both methods, sectioned, stained, and analyzed by an independent observer who was unaware of the processing method.
Results: The study showed a significant decrease in processing time by the microwave method (p-value < .0001).
Conclusion: The present study concluded that the time taken for dehydrating, clearing, and embedding the tissues in paraffin wax was found to be considerably lower than that taken for the conventional method of tissue processing. Chloroform was used here as a clearing agent, and it had desirable effects.
Keywords: Microwave tissue processing, Conventional tissue processing.
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