IJAR.2024.117
Type of Article: Original Research
Volume 12; Issue 2 (June 2024)
Page No.: 8924-8930
DOI: https://dx.doi.org/10.16965/ijar.2024.117
Temporomandibular Joint Histology
Daniela Botero-González .
*1 Assistant professor, Department of Basic Medical Sciences, Faculty of Health Science, Universidad Tecnológica de Pereira, Pereira, Colombia. ORCiD 0000-0002-0156-4997
Corresponding Author: Daniela Botero-González, OD, MSc, PhD., Assistant professor, Department of Basic Medical Sciences, Faculty of Health Science, Universidad Tecnológica de Pereira, Pereira, Colombia. E-Mail: dbotero@utp.edu.co.
ABSTRACT
Background: The temporomandibular joint plays a crucial role in daily oral functions like mastication. A comprehensive understanding of the histological components of these joints proves valuable in deciphering their intricate anatomical structures.
Purpose of the study: This study aimed to compare the histochemical stains of hematoxylin-eosin and Goldner’s trichrome applied to a temporomandibular joint sample using the Wistar Rat as a biomodel.
Results: Complete sagittal slices of murine biomodel hemicranium were evaluated and the comprehensive histological technique involved various steps, including perfusion, post-fixation, decalcification, dehydration, clarification, inclusion, cutting, staining, and assembly. The temporomandibular joint specimens were stained using H&E and Goldner’s trichrome. The histological description included structures such as the external auditory canal, mandibular fossa and condyle, pterygoid muscles, joint capsule, synovial membrane, articular disc, and articular surface. Special cell types like chondrocytes and synovial cells were also identified.
Conclusion: Goldner’s trichrome, an underutilized technique, exhibited superior chromatic richness in differentiating the cellular and tissue components of the temporomandibular joint.
Keywords: Histology, Joint Capsule, Synovial Membrane, Temporomandibular Joint, Temporomandibular Joint Disc, Trichrome Stain, Wistar Rats.
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