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IJAR.2016.486

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Type of Article:  Original Research

Volume 5; Issue 1 (January 2017)

Page No.: 3389-3393

DOI: https://dx.doi.org/10.16965/ijar.2016.486

STRUCTURAL ABERRATIONS OF ‘Y’ CHROMOSOME IN AZOOSPERMIC MALES

Gajanan Laxmanrao Maske 1, Archana Damdharrao Kannamwar *2.

1 Assistant Professor, Department of Anatomy, Shri. V. N. Government Medical College, Yavatmal, India.

*2 Associate Professor, Department of Anatomy, Shri. V. N. Government Medical College, Yavatmal, India.

Address for Correspondence: Dr. Archana Damdharrao Kannamwar, Shri. V. N. Government Medical College, Yavatmal.445001. India. E-Mail: drgajananlmaske@gmail.com

ABSTRACT 
Background: Male factor infertility is a distressing condition that adds to the psychological trauma to majority of couples. Infertility affects about 15% of all couples attempting pregnancy, with male factor identified in approximately half the cases. One of the major contributing factors of failure of sperm production in testis is genetic disturbance. This can be seen either at chromosomal level or at gene level. Chromosomal abnormalities, numerical or structural can occur in somatic cells (mitotic), testicular germ cells (meiotic) or spermatozoa (gametic).

The context and purpose of the study: 30 non-obstructive Azoospermic infertile males from Acharya Vinoba Bhave Rural Hospital, Sawangi (Meghe), Wardha, were selected for present study. From each subject 3 ml venous blood was collected in a sterile bulb with the help of preheparizined syringes. Chromosomal analysis was carried out by conventional as well as Giemsa Trypsin Giemsa (GTG) technique in cytogenetic laboratory, Department of Anatomy, J. N. Medical College, Sawangi (Meghe), Wardha. For each subject, total 25 metaphases i. e.  15 conventional and 10 G-banded metaphases were analyzed. In cases with chromosomal abnormalities, total 45 metaphases i. e. 25 conventional and 20 G-banded metaphases were studied. Selected metaphases were photographed using CCD camera.

Results: Structural aberration of ‘Y’ chromosome was found in 2 subjects. In both these subjects, the ‘q’ arm of ‘Y’ chromosome was long (46, XYq+) as compare to chromosomes of ‘F’ group. The total percentage of 46, XYq+ in present study comes to 6.67%. This was confirmed by G-banding.

Conclusions:  2 subjects (6.67%)   were detected with long ‘q’ arm of ‘Y’ chromosome (46, XYq+) which compares favorably with literatures on the same subject.

Potential implications: Assisted method of reproduction was an option of treatment for infertile males. The same study could be combined with molecular genetic studies to ascertain the chromosomal anomalies at molecular level and thereby proper counseling could be given to infertile couples. This can be of vital role in planning of parenthood.

Key words: Azoospermia, Metaphase, Y Chromosome, q’ arm.

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Cite this article: Gajanan Laxmanrao Maske Archana Damdharrao Kannamwar. STRUCTURAL ABERRATIONS OF ‘Y’ CHROMOSOME IN AZOOSPERMIC MALES. Int J Anat Res 2017;5(1):3389-3393. DOI: 10.16965/ijar.2016.486