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IJAR.2018.457

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Type of Article:  Original

Volume 7; Issue 1.3 (March 2019)

Page No.: 6274-6279

DOI: https://dx.doi.org/10.16965/ijar.2018.457

STRUCTURAL CHANGES OBSERVED IN MICE LIVER DUE TO CHRONIC EXPOSURE OF 1900-2200 MHZ RADIATIONS EMITTED FROM MOBILE PHONE

Akintunde O.Wasiu *, Abdulrahman Abdulfatai.

Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences Ladoke Akintola University of Technology, Ogbomoso, Nigeria.

Corresponding Author’s Address: Dr. Akintunde O.Wasiu, Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences Ladoke Akintola University of Technology, Ogbomoso, Nigeria. Mobile +2348033977181 E-Mail: owakintunde@lautech.edu.ng

ABSTRACT:

Herbicide(s) commonly known as weedkillers, are chemical substances used to control unwanted plants. Paraquat (PQ) (1, 1-dimethyl 4, 4- bipyridillium dichloride) is the active component of the herbicide (Parae Force) used in this study.

This study was performed on 25 male Wistar rats that were divided into 5 groups (A, B, C, D and E), Group A served as the control group that received only distilled water, Group B, C, D & E served as the treated groups that received 25%, 50%, 75%, & 100% leachate of Paraquat dichloride concentration respectively for 8 weeks.

This study investigated the effect of paraquat dichloride on the testes, sperm characteristics and body weight in adult male Wistar rats. Histological analysis of the testes as well as semen analysis was carried out.

Histomorphometry study of the testes showed deformed seminiferous tubules with irregular shape and spermatogenesis was arrested. Interstitial space was comparatively loose and disrupted Leydig cells are also visible. Seminiferous tubules also exhibiting degenerated basement membrane along with degenerated interstitial cells, degenerated spermatogonia and damaged Sertoli cells. Consequently, the sperm count from the Paraquat dichloride exposed semen revealed significant decreased results, the sperm morphology and sperm progressivity were also affected negatively.

In conclusion, the present study indicates that Paraquat dichloride demonstrated toxic effects on male reproductive parameters.

Key words: Paraquat dichloride, Testis, Sperm parameter, Rat.

REFERENCES

  1. Lambert CE, Bondy SC. Effects of MPTP, and paraquat on mitochondrial potential and oxidative stress. Life sciences 1989;44(18):1277-84.
  2. US EPA. Office Of Prevention, Pesticides and Toxic Substances Registraion Eligibility Decision (RED): trifluralin. Washington D.C. April 1996.
  3. Hamay Atkhah V, Najafiyan M, Farzam M, Kargar H. Effects of the Paraquat Herbicide on Oogenesis and Ovary Structure of Wistar Rat strain. Advances in Environmental Biology. 2004;6(3):1006-12.
  4. Jafarinejad S, Ghazi-khansari M. Evaluation of the Effects of Captopril on Cytotoxicity of Paraquat in G292 Cell Line. Journal of Isfahan Medical School. 2017;30(196): 12-20.
  5. Smith LL. Mechanism of paraquat toxicity in lung and its relevance to treatment. Hum Toxicol. 1987;6:31-36.
  6. Hawksworth GH, Bennett PN, Davies DS. Kinetics of paraquat elimination in the dog. Toxicol, Appl., Pharmacol. 1981;57:139.
  7. Smith LL, Wright A, Wyatt I, and Rose MS. Effective treatment for paraquat poisoning in rats and its relevance to the treatment of paraquat poisoning in man. Br. Med. J. 1974;4.
  8. Bennett PN, Davies DS, Hawksworth GH. In vitro absorption studies with paraquat and diquat in the dog. Br J Pharmacol. 1976;58:284.
  9. Wester RC, Maibach HI, Bucks DA, and Aufrere MB. In vivo percutaneous absorption of paraquat from hand leg and forearm of humans. J Toxicol. Environ. Hlth. 1984;14.
  10. WHO, 1999. Interpretation of semen analysis using WHO 1999 and WHO 2010 reference values: Abnormal become Normal.
  11. Zain A. The evaluation of the toxic effect of paraquat and its mechanism of action on reproductive system of male rats: Thesis. Universiti Sains Malaysia, Penang 2007. http://eprints. usm.
  12. Deepananda Ashoka KHM and De Silva WAJ. Mechanism of Paraquat Action shows Interference in Spermatogenesis and Epididymal Maturation of Sperm in Mice. Journal of Biology, 2013;1(3):67-76.
  13. Clark, D.G, McElligot, T.F and Weston Hurst, E. The Toxicity of paraquat. Brit. J. Industr. Med. 1966;26:126.
  14. Butler, C and Kleinerman, J. Paraquat in the rabbit, Br. J. ind. Med. 1971;28:67-71.
  15. Ranjbar A, Mohsenzadeh F, Chehregani A, Khajavi F, Zijoud S, Ghasemi H. Ameliorative effect of Matricaria chamomilla. L on paraquat: Induced oxidative damage in lung rats. Pharmacognosy Research. 2014; 6(3):199.
  16. Johnson, E., Wayne, S., Cook, K., Nagoshi, R. Phenotypic characterization of no ovaries (nov). A. Dros. Res. Conf. 1992;33: 56.
  17. Srikanth V, Malini T, Arunakaran J, Govindarajulu P, Balasubramanian K. Effect of ethanol treatment on epididymal secretory product and sperm maturation in albino rats. Pharmacol. 1999;288(2):509-519.
  18. Ohi, H., Shimizu, M., Obata, M., Funabiki, T and Yano, S. Poly[[µ3-tris(4-phenyl-1,2,3-triazol-1-y)ethyl]amine}silver(1)]hexafluoridophosphate]. Acta Cryst. Section E 2008.
  19. Narayana K, Prashanthi N, Nayanatara A, Kumar HH, Abhilash K, Bairy KL. 2006. Neonatal methyl parathion exposure affects growth and functions of the male reproductive system in the adult rat. Folia Morphol (Warsz)2006;65(1): 26-33.
  20. Ngoula F, Watcho P, Dongmo MC, Kenfac A, Kamtchouing P, Tchoumboue J. Effects of primphos-methyl (an organophosphate insecticide) on the fertility of adult male rats. Afr Health Sci. 2007;7(1): 3-9.
  21. Bone W, Jone AR, Morin C, Nieschlag E, Cooper TG. Susceptibility of glycolytic enzyme activities and motility of spermatozoa from rat, mouse and human to inhibition by proven and putative chlorinated antifertility compounds in vitro. J Androl 2001;22(3):464-470.
  22. Joshi SC, Mathur R, Gilati N. Testicular toxicity of chlorpyrifos (an organophosphate pesticide) in albino rat. Toxicol Ind Health 2007;23: 439-444.
  23. Gage, J.C. 1968a, Toxicity of Paraquat and diquat aerosols generated by a size selective cyclone: Effect of particle size distribution. Br. J. Ind. Med. 1968a;95:304 – 314.
  24. Nienhaus H and Ehrenfeld 1971. Pathogenesis of lung disease in paraquat poisoning. Beitr. Path. Anat, 1971;142:244–267.
  25. Bronkhorst F.B., Van Daal, J.M and Tan, H.D. Fatal outcome of a case of paraquat (Gramoxone) poisoning. Ned. Tijdschr.Geneesk, 1968;112:310-313.

Cite this article: Akintunde O.Wasiu, Abdulrahman Abdulfatai. TOXIC EFFECTS OF PARAQUAT DICHLORIDE LEACHATE ON TESTES AND SPERM PARAMETERS OF MALE WISTAR RATS. Int J Anat Res 2019;7(1.3):6274-6279. DOI: 10.16965/ijar.2018.457