Welcome to International Journal of Anatomy and Research

Type of Article: Original Research

Year: 2016 | Volume 4 | Issue 1 | Page No. 1977-1980

Date of Publication: 29-02-2016

DOI: http://dx.doi.org/10.16965/ijar.2016.124

HISTOLOGICAL INVESTIGATIONS ON KIDNEY OF SILDENAFIL CITRATE (EDEGRA) TREATED ALBINO MICE

K. V. P. Suriyakumari *¹, R. Udayakumar ², T. Ruba ².

^*1 Department of Anatomy, Sri Manakula Vinayagar Medical College and Hospital, Madagadipet, Puducherry, India.
² DDE Study Centre, Annamalai University, Villupuram, Tamil Nadu, India.

Address: Dr. K. V. P. Suriyakumari, Professor and Head, Department of Anatomy, Sri Manakula Vinayagar Medical College and Hospital, Madagadipet, Puducherry- 605107, India.
E-Mail: suriyaudhay@gmail.com

Abstract

Background: Edegra (Sildenafil citrate), an effective oral therapy for Erectile Dysfunction, being the citrate salt of Sildenafil is a selective inhibitor of cGMP- specific phosphodiesterase type 5 (PDE 5).
Aim: To study the long- term impact of Sildenafil citrate on the histological components of kidney of Albino mice.
Materials and Methods: Thirty six healthy male Wistar Albino mice were selected on weight basis and divided into six groups (S1, S2, S3, S4, S5 and S6), each consisting of six animals in it. Here, Group S1 and S5 served as the control while the rest of the four groups (i.e. S2, S3, S4 and S6) served as the experimental ones. Group S1 animals were treated with a single dosage of conductivity water while the experimental animals, namely, S2, S3 and S4 were treated with a single dosage of the chosen drug (@ 1µg/g body wt.) and sacrificed after 1 hr., 4 hrs. and 24 hrs. of the last dosage. Group S5 animals were treated daily with a single dosage of conductivity water while the group S6 animals were treated daily with a single dosage of the chosen drug (@ 1µg/g body wt.) for all the 15 days and then sacrificed after 4 hrs. of the last dosage. Vertical ventral midline incision was made in the abdominal wall to collect both the kidney samples. The organs were preserved in 10% formalin saline, processed and stained with Eosin and Haematoxylin stains.
Results: Histopathological observations have been made on the collected kidney samples of Albino mice treated with Sildenafil citrate (Edegra). Increase in the periglomerular spaces, shrinkage of Glomeruli, increased number of vacuolated cells and reduced number of Glomeruli in cortical area due to destruction of Glomeruli have been strikingly noticed for long- term drug treated samples.
Conclusion: Sildenafil citrate (Edegra), if administered for a long- term, will produce drastic impacts on the histoarchitecture and vital functions of kidney of Albino mice.
Key words: Albino mice, Kidney, Edegra, Histology, Vacuolated cells.

References

1. Gauv AI. Erectile dysfunction- Are you prepared to discuss it? Postraduate Medicine, 1995; 97: 127-143.
2. Burnett AL. Erectile dysfunction: a practical approach for the primary care. Geriatrics, 1998; 53(2): 46-48.
3. Korenman SG. New insights into erectile dysfunction: a practical approach. American Journal of Medicine, 1998; 105(2): 135-144.
4. Benet AE, Melman A. The epidemiology of erectile dysfunction. Urologic Clinics of North America, 1995; 22(4): 699-709.
5. Feldman HA, Goldstein I, Hatzichristou DG, Krane RJ, McKinlay JB. Impotence and its medical and psychological correlates: results of the Massachusetts Male Aging Study. J Urol., 1994; 151(1): 54-61.
6. McCulloch DK, Young RJ, Prescott RJ, Campbell IW, Clarke BF. The natural history of impotence in diabetic men. Diabetologia 1984; 26(6): 437-440.
7. De Berardis G, Pellegrint F, Francioso M, Belfiglio M, Di Nardo B, Greenfield S et al. Identifying patients with type 2 diabetes with a higher likelihood of erectile dysfunction: the role of the interaction between clinical and psychological factors. J. Urol., 2003; 169(4): 1422-1428.
8. Naliboff BD, Rosenthal M. Effects of Age on Complications in Adult Onset Diabetes. Journal of the American Geriatrics Society, 1989; 37(9): 838-842.
9. Eardley I, Brooks J, Yates PK, Ellis P, Boolell M. Sildenafil citrate (VIAGRA): an oral treatment for erectile dysfunction with activity for upto four hours duration. International Journal of Clinical Practice, 1999 Suppl June; 102: 32-34.
10. Eardley I, Ellis P, Boolell M, Wulff M. Onset and duration of Sildenafil citrate for the treatment of Erectile dysfunction. British Journal of Clinical Pharmacology, 2002; 53 (Suppl 1): 615-655.
11. Sakr SA, El-Messedy FA and Abdel-Samei HA. Histopathological and histochemical effects of gibberellin A3 on the kidney of albino rats. J Egypt Germ Soc Zool, 2002; 38: 1- 10.
12. Gamze Ozbay, Nurgayat Barlas, Diirdane Kolankaya. Histopathological effects of the residual Meneb and Zineb in the lettuces on the liver and kidney of Albino mice. Journal of Islamic Academy of Sciences, 1991; 4(4): 336-339.
13. Bakr SM, Moussa EM, Khater Esh. Cytotoxic evaluation of Gibberellins A3 in swiss Albino mice. J. Union Arab. Biol., 1999; 11: 345-351.
14. Abdeen AM, Amer TA, El- Habibi EM, Kamal EM. Histological and histochemical studies on the effect of the fenvalerate insecticide on some organs of the Albino mice. J. Union Arab Biol., 1994; 2A: 129-166.
15. Bhuvarahamurthy V, Balasubramanian N, Govindasamy S. Effect of Radiotherapy and Chemotherapy on circulating antioxidant system of human uterine cervical carcinoma. Mol Cell Biochem, 1996; 158: 17-23.
16. Sherlock S, Dooley J. In: Disease of the liver and biliary system. 9th edition. Blackwell Scientific Publication, Cambridge, London, 1993, 649.
17. Zhang LY, Wang CX. Histopathological and histochemical studies on toxic effects of Brodifacoun in mouse liver. Acta Acad Med Sci., 1984; 6(9): 386-388.

K. V. P. Suriyakumari, R. Udayakumar, T. Ruba. HISTOLOGICAL INVESTIGATIONS ON KIDNEY OF SILDENAFIL CITRATE (EDEGRA) TREATED ALBINO MICE. Int J Anat Res 2016;4(1):1977-1980. DOI: 10.16965/ijar.2016.124